Journal article
Quantitative assessment of Tn antigen in breast tissue micro-arrays using CdSe aqueous quantum dots
Biomaterials, v 35(9), pp 2971-2980
Mar 2014
PMID: 24411673
Abstract
In this study, we examined the use of CdSe aqueous quantum dots (AQDs) each conjugated to three streptavidin as a fluorescent label to image Tn antigen expression in various breast tissues via a sandwich staining procedure where the primary monoclonal anti-Tn antibody was bound to the Tn antigen on the tissue, a biotin-labeled secondary antibody was bound to the primary anti-Tn antibody, and finally the streptavidin-conjugated AQDs were bound to the biotin on the secondary antibody. We evaluated the AQD staining of Tn antigen on tissue microarrays consisting of 395 cores from 115 cases including three tumor cores and one normal-tissue core from each breast cancer case and three tumor cores from each benign case. The results indicated AQD-Tn staining was positive in more than 90% of the cells in the cancer cores but not the cells in the normal-tissue cores and the benign tumor cores. As a result, AQD-Tn staining exhibited 95% sensitivity and 90% specificity in differentiating breast cancer against normal breast tissues and benign breast conditions. These results were better than the 90% sensitivity and 80% specificity exhibited by the corresponding horse radish peroxidase (HRP) staining using the same antibodies on the same tissues and those of previous studies that used different fluorescent labels to image Tn antigen. In addition to sensitivity and specificity, the current AQD-Tn staining with a definitive threshold was quantitative.
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Details
- Title
- Quantitative assessment of Tn antigen in breast tissue micro-arrays using CdSe aqueous quantum dots
- Creators
- Giang H T Au - School of Biomedical Engineering, Science and Health System, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USALinette Mejias - Department of Pathology and Laboratory Medicine, College of Medicine, Drexel University, 245 N. 15th Street, Philadelphia, PA 19102, USAVanlila K Swami - Department of Pathology and Laboratory Medicine, College of Medicine, Drexel University, 245 N. 15th Street, Philadelphia, PA 19102, USAAri D Brooks - Department of Surgery, College of Medicine, Drexel University, 245 N. 15th Street, Philadelphia, PA 19102, USAWan Y Shih - School of Biomedical Engineering, Science and Health System, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USAWei-Heng Shih - Department of Materials Science and Engineering, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USA. Electronic address: shihwh@drexel.edu
- Publication Details
- Biomaterials, v 35(9), pp 2971-2980
- Publisher
- Elsevier; Netherlands
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering; Surgery
- Web of Science ID
- WOS:000332188900040
- Scopus ID
- 2-s2.0-84895016753
- Other Identifier
- 991014877694204721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials