Journal article
Quantitative, directional measurement of electric field heterogeneity in the active site of ketosteroid isomerase
Proceedings of the National Academy of Sciences - PNAS, v 109(6), pp E299-E308
07 Feb 2012
PMID: 22308339
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Understanding the electrostatic forces and features within highly heterogeneous, anisotropic, and chemically complex enzyme active sites and their connection to biological catalysis remains a longstanding challenge, in part due to the paucity of incisive experimental probes of electrostatic properties within proteins. To quantitatively assess the landscape of electrostatic fields at discrete locations and orientations within an enzyme active site, we have incorporated site-specific thiocyanate vibrational probes into multiple positions within bacterial ketosteroid isomerase. A battery of X-ray crystallographic, vibrational Stark spectroscopy, and NMR studies revealed electrostatic field heterogeneity of 8 MV/cm between active site probe locations and widely differing sensitivities of discrete probes to common electrostatic perturbations from mutation, ligand binding, and pH changes. Electrostatic calculations based on active site ionization states assigned by literature precedent and computational pK
a
prediction were unable to quantitatively account for the observed vibrational band shifts. However, electrostatic models of the D40N mutant gave qualitative agreement with the observed vibrational effects when an unusual ionization of an active site tyrosine with a pK
a
near 7 was included. UV-absorbance and
13
C NMR experiments confirmed the presence of a tyrosinate in the active site, in agreement with electrostatic models. This work provides the most direct measure of the heterogeneous and anisotropic nature of the electrostatic environment within an enzyme active site, and these measurements provide incisive benchmarks for further developing accurate computational models and a foundation for future tests of electrostatics in enzymatic catalysis.
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Details
- Title
- Quantitative, directional measurement of electric field heterogeneity in the active site of ketosteroid isomerase
- Creators
- Aaron T. Fafarman - Department of ChemistryPaul A. Sigala - Department of Biochemistry, andJason P. Schwans - Department of Biochemistry, andTimothy D. Fenn - Stanford UniversityDaniel Herschlag - Department of Biochemistry, andSteven G. Boxer - Department of Chemistry
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 109(6), pp E299-E308
- Series
- PNAS Plus
- Publisher
- National Academy of Sciences
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000299925000004
- Scopus ID
- 2-s2.0-84857125236
- Other Identifier
- 991020834713104721
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- Web of Science research areas
- Biochemistry & Molecular Biology