Journal article
Quartz crystal microbalance based biosensor for rapid and sensitive detection of maize chlorotic mottle virus
Analytical methods, v 6(13), pp 4530-4536
01 Jan 2014
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In this work, we report a biosensor based on a quartz crystal microbalance ( QCM) for the selective and sensitive detection of maize chlorotic mottle virus (MCMV). A mixture of 10 : 1 3-mercaptopropanoic acid and 11-mercaptoundecanoic acid was applied on gold surfaces of QCM crystals to form a selfassembled monolayer, and an anti-MCMV antibody was crosslinked on the surface for specific recognition of MCMV. The frequency change of the QCM crystal is a function of the concentration of MCMV with a response range from 250 ng mL(-1) to 10 mg mL(-1). The standard curve is R2 0.997, indicating a high reproducibility in MCMV detection. The detection limit is approximately 250 ng mL(-1), which is similar to that of the existing enzyme-linked immunosorbent assay (ELISA) method. The QCM sensor showed highly specific and sensitive recognition for both purified MCMV and crude extracts from corn leaf samples.
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Details
- Title
- Quartz crystal microbalance based biosensor for rapid and sensitive detection of maize chlorotic mottle virus
- Creators
- Xin Huang - Chinese Academy of Inspection and QuarantineJianmin Xu - Chinese Academy of Inspection and QuarantineHai-Feng Ji - Drexel UniversityGuifen Li - Chinese Academy of Inspection and QuarantineHongjun Chen - Chinese Academy of Inspection and Quarantine
- Publication Details
- Analytical methods, v 6(13), pp 4530-4536
- Publisher
- Royal Soc Chemistry
- Number of pages
- 7
- Grant note
- 20141004 / AQSIQ Non- profit Industry Research Projects 2013ZX08012-001 / National Science and Technology Specific Projects
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000337763200009
- Scopus ID
- 2-s2.0-84902492796
- Other Identifier
- 991019330629104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Analytical
- Food Science & Technology
- Spectroscopy