Files and links (2)
Published, Version of Record (VoR)CC BY V4.0, Open
Journal article
RECQ1 possesses DNA branch migration activity
The Journal of biological chemistry, v 283(29), pp 20231-20242
18 Jul 2008
PMID: 18495662
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
RecQ helicases are essential for the maintenance of genome stability. Five members of the RecQ family have been found in humans, including RECQ1, RECQ5, BLM, WRN, and RECQ4; the last three are associated with human diseases. At this time, only BLM and WRN helicases have been extensively characterized, and the information on the other RecQ helicases has only started to emerge. Our current paper is focused on the biochemical properties of human RECQ1 helicase. Recent cellular studies have shown that RECQ1 may participate in DNA repair and homologous recombination, but the exact mechanisms of how RECQ1 performs its cellular functions remain largely unknown. Whereas RECQ1 possesses poor helicase activity, we found here that the enzyme efficiently promotes DNA branch migration. Further analysis revealed that RECQ1 catalyzes unidirectional three- stranded branch migration with a 3'-> 5' polarity. We show that this RECQ1 activity is instrumental in specific disruption of joint molecules (D- loops) formed by a 5' single- stranded DNA invading strand, which may represent dead end intermediates of homologous recombination in vivo. The newly found enzymatic properties of the RECQ1 helicase may have important implications for the function of RECQ1 in maintenance of genomic stability.
Metrics
Details
- Title
- RECQ1 possesses DNA branch migration activity
- Creators
- Dmitry V. Bugreev - Drexel UniversityRobert M. Brosh - National Institutes of HealthAlexander V. Mazin - Drexel University
- Publication Details
- The Journal of biological chemistry, v 283(29), pp 20231-20242
- Publisher
- Amer Soc Biochemistry Molecular Biology Inc
- Number of pages
- 12
- Grant note
- ZIAAG000741 / NATIONAL INSTITUTE ON AGING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA) CA 100839 / NCI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) R01CA100839 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) Intramural NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000257565300039
- Scopus ID
- 2-s2.0-50649118135
- Other Identifier
- 991019168026304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology