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Journal article
RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex
The EMBO journal, v 24(11), pp 2024-2033
01 Jun 2005
PMID: 15889139
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
SGS1 encodes a DNA helicase whose homologues in human cells include the BLM, WRN, and RECQ4 genes, mutations in which lead to cancer‐predisposition syndromes. Clustering of synthetic genetic interactions identified by large‐scale genetic network analysis revealed that the genetic interaction profile of the gene RMI1 (RecQ‐mediated genome instability, also known as NCE4 and YPL024W) was highly similar to that of SGS1 and TOP3, suggesting a functional relationship between Rmi1 and the Sgs1/Top3 complex. We show that Rmi1 physically interacts with Sgs1 and Top3 and is a third member of this complex. Cells lacking RMI1 activate the Rad53 checkpoint kinase, undergo a mitotic delay, and display increased relocalization of the recombination repair protein Rad52, indicating the presence of spontaneous DNA damage. Consistent with a role for RMI1 in maintaining genome integrity, rmi1Δ cells exhibit increased recombination frequency and increased frequency of gross chromosomal rearrangements. In addition, rmi1Δ strains fail to fully activate Rad53 upon exposure to DNA‐damaging agents, suggesting that Rmi1 is also an important part of the Rad53‐dependent DNA damage response.
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Details
- Title
- RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex
- Creators
- Michael Chang - University of TorontoMohammed Bellaoui - University of TorontoChaoying Zhang - University of TorontoRidhdhi Desai - University of TorontoPavel Morozov - Columbia UniversityLissette Delgado-Cruzata - Columbia UniversityRodney Rothstein - Columbia UniversityGreg A Freyer - Columbia UniversityCharles Boone - University of TorontoGrant W Brown - University of Toronto
- Publication Details
- The EMBO journal, v 24(11), pp 2024-2033
- Publisher
- John Wiley & Sons, Ltd
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000229588100012
- Scopus ID
- 2-s2.0-21244434850
- Other Identifier
- 991022035116304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology