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Rab14 regulates apical targeting in polarized epithelial cells
Journal article   Open access

Rab14 regulates apical targeting in polarized epithelial cells

Khameeka N Kitt, Delia Hernández-Deviez, Sarah D Ballantyne, Elias T Spiliotis, James E Casanova and Jean M Wilson
Traffic (Copenhagen, Denmark), v 9(7), pp 1218-1231
Jul 2008
DOI: https://doi.org/10.1111/j.1600-0854.2008.00752.x
PMID: 18429929
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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https://doi.org/10.1111/j.1600-0854.2008.00752.xView
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Abstract

rab GTP-Binding Proteins - metabolism Cell Line Glutathione Transferase - metabolism Rats Cytoplasm - metabolism Endosomes - metabolism Protein Transport Two-Hybrid System Techniques rab GTP-Binding Proteins - physiology Animals GTP Phosphohydrolases - metabolism Dogs Golgi Apparatus - metabolism Mutation Epithelial Cells - cytology
Epithelial cells display distinct apical and basolateral membrane domains, and maintenance of this asymmetry is essential to the function of epithelial tissues. Polarized delivery of apical and basolateral membrane proteins from the trans Golgi network (TGN) and/or endosomes to the correct domain requires specific cytoplasmic machinery to control the sorting, budding and fission of vesicles. However, the molecular machinery that regulates polarized delivery of apical proteins remains poorly understood. In this study, we show that the small guanosine triphosphatase Rab14 is involved in the apical targeting pathway. Using yeast two-hybrid analysis and glutathione S-transferase pull down, we show that Rab14 interacts with apical membrane proteins and localizes to the TGN and apical endosomes. Overexpression of the GDP mutant form of Rab14 (S25N) induces an enlargement of the TGN and vesicle accumulation around Golgi membranes. Moreover, expression of Rab14-S25N results in mislocalization of the apical raft-associated protein vasoactive intestinal peptide/MAL to the basolateral domain but does not disrupt basolateral targeting or recycling. These data suggest that Rab14 specifically regulates delivery of cargo from the TGN to the apical domain.

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Collaboration types
Domestic collaboration
Web of Science research areas
Cell Biology
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