Journal article
Rad51 protein stimulates the branch migration activity of Rad54 protein
The Journal of biological chemistry, v 283(36), pp 24698-24706
05 Sep 2008
PMID: 18617519
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The Rad51 and Rad54 proteins play important roles during homologous recombination in eukaryotes. Rad51 forms a nucleoprotein filament on single-stranded DNA and performs the initial steps of double strand break repair. Rad54 belongs to the Swi2/Snf2 family of ATP-dependent DNA translocases. We previously showed that Rad54 promotes branch migration of Holliday junctions. Here we find that human Rad51 (hRad51) significantly stimulates the branch migration activity of hRad54. The stimulation appears to be evolutionarily conserved, as yeast Rad51 also stimulates the branch migration activity of yeast Rad54. We further investigated the mechanism of this stimulation. Our results demonstrate that the stimulation of hRad54-promoted branch migration by hRad51 is driven by specific protein-protein interactions, and the active form of the hRad51 filament is more stimulatory than the inactive one. The current results support the hypothesis that the hRad51 conformation state has a strong effect on interaction with hRad54 and ultimately on the function of hRad54 in homologous recombination.
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Details
- Title
- Rad51 protein stimulates the branch migration activity of Rad54 protein
- Creators
- Matthew J Rossi - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102-1192, USAAlexander V Mazin
- Publication Details
- The Journal of biological chemistry, v 283(36), pp 24698-24706
- Publisher
- ASBMB Publications / Elsevier; United States
- Grant note
- CA100839 / NCI NIH HHS MH084119 / NIMH NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000258820000039
- Scopus ID
- 2-s2.0-54049139260
- Other Identifier
- 991014877953004721
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- Web of Science research areas
- Biochemistry & Molecular Biology