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Rad52 Inverse Strand Exchange Drives RNA-Templated DNA Double-Strand Break Repair
Journal article   Open access   Peer reviewed

Rad52 Inverse Strand Exchange Drives RNA-Templated DNA Double-Strand Break Repair

Olga M Mazina, Havva Keskin, Kritika Hanamshet, Francesca Storici and Alexander V Mazin
Molecular cell, v 67(1), 29.e3
06 Jul 2017
DOI: https://doi.org/10.1016/j.molcel.2017.05.019
PMID: 28602639
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1016/j.molcel.2017.05.019View
Published, Version of Record (VoR)Open Access (Publisher-Specific),  Open

Abstract

DNA Breaks, Double-Stranded DNA Repair DNA, Fungal - genetics DNA, Fungal - metabolism DNA, Single-Stranded - genetics DNA, Single-Stranded - metabolism Humans Mutation Nucleic Acid Heteroduplexes Protein Binding Rad52 DNA Repair and Recombination Protein - genetics Rad52 DNA Repair and Recombination Protein - metabolism RNA, Fungal - genetics RNA, Fungal - metabolism Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Templates, Genetic Time Factors
RNA can serve as a template for DNA double-strand break repair in yeast cells, and Rad52, a member of the homologous recombination pathway, emerged as an important player in this process. However, the exact mechanism of how Rad52 contributes to RNA-dependent DSB repair remained unknown. Here, we report an unanticipated activity of yeast and human Rad52: inverse strand exchange, in which Rad52 forms a complex with dsDNA and promotes strand exchange with homologous ssRNA or ssDNA. We show that in eukaryotes, inverse strand exchange between homologous dsDNA and RNA is a distinctive activity of Rad52; neither Rad51 recombinase nor the yeast Rad52 paralog Rad59 has this activity. In accord with our in vitro results, our experiments in budding yeast provide evidence that Rad52 inverse strand exchange plays an important role in RNA-templated DSB repair in vivo.

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102 citations in Web of Science
102 citations in Scopus

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
Cell Biology
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