Journal article
Rad54 Protein Is Targeted to Pairing Loci by the Rad51 Nucleoprotein Filament
Molecular cell, v 6(3), pp 583-592
2000
PMID: 11030338
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Rad51 and Rad54 proteins are important for the repair of double-stranded DNA (dsDNA) breaks by homologous recombination in eukaryotes. Rad51 assembles on single-stranded DNA (ssDNA) to form a helical nucleoprotein filament that performs homologous pairing with dsDNA; Rad54 stimulates this pairing substantially. Here, we demonstrate that Rad54 acts in concert with the mature Rad51-ssDNA filament. Enhancement of DNA pairing by Rad54 is greatest at an equimolar ratio relative to Rad51 within the filament. Reciprocally, the Rad51-ssDNA filament enhances both the dsDNA-dependent ATPase and the dsDNA unwinding activities of Rad54. We conclude that Rad54 participates in the DNA homology search as a component of the Rad51-nucleoprotein filament and that the filament delivers Rad54 to the dsDNA pairing locus, thereby linking the unwinding of potential target DNA with the homology search process.
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Details
- Title
- Rad54 Protein Is Targeted to Pairing Loci by the Rad51 Nucleoprotein Filament
- Creators
- Alexander V Mazin - Division of Biological Sciences, Sections of Microbiology and of Molecular and Cellular Biology, University of California, Davis, Davis, California 95616, USACarole J Bornarth - Division of Biological Sciences, Sections of Microbiology and of Molecular and Cellular Biology, University of California, Davis, Davis, California 95616, USAJachen A Solinger - Division of Biological Sciences, Sections of Microbiology and of Molecular and Cellular Biology, University of California, Davis, Davis, California 95616, USAWolf-Dietrich Heyer - Division of Biological Sciences, Sections of Microbiology and of Molecular and Cellular Biology, University of California, Davis, Davis, California 95616, USAStephen C Kowalczykowski - Division of Biological Sciences, Sections of Microbiology and of Molecular and Cellular Biology, University of California, Davis, Davis, California 95616, USA
- Publication Details
- Molecular cell, v 6(3), pp 583-592
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000089578600008
- Scopus ID
- 2-s2.0-0033634677
- Other Identifier
- 991014877817204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology