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Rad54, the motor of homologous recombination
Journal article   Open access   Peer reviewed

Rad54, the motor of homologous recombination

Alexander V Mazin, Olga M Mazina, Dmitry V Bugreev and Matthew J Rossi
DNA repair, v 9(3), pp 286-302
02 Mar 2010
PMID: 20089461
url
https://doi.org/10.1016/j.dnarep.2009.12.006View
Published, Version of Record (VoR) Open

Abstract

DNA double-strand break repair Snf2 proteins Rad54 protein Homologous recombination Branch migration RecQ helicases ATP-dependent DNA translocation Holliday junctions
Homologous recombination (HR) performs crucial functions including DNA repair, segregation of homologous chromosomes, propagation of genetic diversity, and maintenance of telomeres. HR is responsible for the repair of DNA double-strand breaks and DNA interstrand cross-links. The process of HR is initiated at the site of DNA breaks and gaps and involves a search for homologous sequences promoted by Rad51 and auxiliary proteins followed by the subsequent invasion of broken DNA ends into the homologous duplex DNA that then serves as a template for repair. The invasion produces a cross-stranded structure, known as the Holliday junction. Here, we describe the properties of Rad54, an important and versatile HR protein that is evolutionarily conserved in eukaryotes. Rad54 is a motor protein that translocates along dsDNA and performs several important functions in HR. The current review focuses on the recently identified Rad54 activities which contribute to the late phase of HR, especially the branch migration of Holliday junctions.

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Web of Science research areas
Genetics & Heredity
Toxicology
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