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Journal article
Rapid AAV-Neutralizing Antibody Determination with a Cell-Binding Assay
Molecular therapy. Methods & clinical development, v 13, pp 40-46
29 Nov 2018
PMID: 30623003
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Recombinant adeno-associated virus (rAAV) has been developed as a successful vector for both basic research and human gene therapy. However, neutralizing antibodies (NAbs) against AAV capsids can abolish AAV infectivity on target cells, reducing the transduction efficacy. Absence of AAV NAb has become a prerequisite qualification for patients enrolled in gene therapy trials. Nevertheless, accurate assessment of AAV NAb has remained a challenging task. Here we developed a rapid assay based on the observations that AAV NAb inhibits rAAV binding to the host cell surface and NAb titers are negatively related to the amount of AAV genomes binding to the target cells. By quantifying the AAV genome on the target cells in the presence of anti-sera, AAV NAb titers can be accurately determined. The titer determined by this assay correlates well with the classical transduction-based assays. A major advantage of this method is that it can be carried out with a 30-min binding assay without the lengthy wait for a transduction outcome. This assay is independent of transduction performance of AAV serotype in the target cells. Therefore, the AAV cell-binding assay for NAb determination offers an alternative method for
in vivo
NAb assay.
Metrics
Details
- Title
- Rapid AAV-Neutralizing Antibody Determination with a Cell-Binding Assay
- Creators
- Ping Guo - Huaqiao UniversityJunping Zhang - Huaqiao UniversityMatthew Chrzanowski - Temple UniversityJianhe Huang - Temple UniversityHelen Chew - Temple UniversityJenni A. Firrman - Temple UniversityNianli Sang - Drexel UniversityYong Diao - Huaqiao UniversityWeidong Xiao - Temple University
- Publication Details
- Molecular therapy. Methods & clinical development, v 13, pp 40-46
- Publisher
- American Society of Gene & Cell Therapy
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000471284200004
- Scopus ID
- 2-s2.0-85059301515
- Other Identifier
- 991019167839704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Medicine, Research & Experimental