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Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor
Journal article   Peer reviewed

Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor

Chang Zeng, Xin Huang, Jiangmin Xu, Guifen Li, Jie Ma, Hai-Feng Ji, Shuifang Zhu and Hongjun Chen
Analytical biochemistry, v 440(1), pp 18-22
01 Sep 2013
DOI: https://doi.org/10.1016/j.ab.2013.04.026
PMID: 23660014
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Biosensor Maize chlorotic mottle virus Surface plasmon resonance Virus detection
We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maize chlorotic mottle virus (MCMV). 11-Mercaptoundecanoic acid was applied on a gold surface to form a self-assembled monolayer, and a layer of anti-MCMV antibody was crosslinked on the surface for specific recognition of MCMV. The effects of coupling reaction time and antibody concentration on detection sensitivity were studied. The coverage mass change is a function of the concentration of MCMV with a dynamic range from 1 to 1000ppb. The detection limit is approximately 1ppb, which is approximately two orders of magnitude higher than that of the existing enzyme-linked immunosorbent assay (ELISA) method. The developed SPR sensor showed highly specific recognition for both purified MCMV and crude extracts from real-world samples.

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27 citations in Web of Science
39 citations in Scopus

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UN Sustainable Development Goals (SDGs)

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#2 Zero Hunger
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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Biochemical Research Methods
Biochemistry & Molecular Biology
Chemistry, Analytical
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