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Rapid assessment of Escherichia coli by growth rate on piezoelectric-excited millimeter-sized cantilever (PEMC) sensors
Journal article   Peer reviewed

Rapid assessment of Escherichia coli by growth rate on piezoelectric-excited millimeter-sized cantilever (PEMC) sensors

Andrew J Detzel, Gossett A Campbell and Raj Mutharasan
Sensors and actuators. B, Chemical, v 117(1), pp 58-64
2006
DOI: https://doi.org/10.1016/j.snb.2005.10.045
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Growth rate Detection Resonant frequency Food contamination E. coli
A piezoelectric-excited millimeter sized glass cantilever with a 1 mm 2 sensing surface was fabricated for the purpose of detecting the growth of Escherichia coli ( E. coli) JM101 in real-time. The resonance characteristic of the fundamental mode in air was 31.08 ± 0.01 kHz. Upon the addition of the agar film to the cantilever sensing surface the fundamental resonance frequency decreased by 2 ± 0.01 kHz. Upon subsequent inoculation of E. coli JM101, the fundamental frequency decreases an additional 0.1 ± 0.01 kHz. The E. coli was grown at 29 °C in a temperature-controlled chamber. The E. coli went through a lag phase of 1 h followed by a continuous decrease in resonant frequency due to growth of the bacteria. The total change in resonant frequency during the growth phase was 5.08 ± 0.01 kHz. After 6 h, the resonant frequency reached a constant value as growth ended. A model was developed to relate resonant frequency to specific growth rate ( μ). The E. coli exhibited exponential growth rate of 1.31 ± 0.05 h −1 and a late exponential growth rate of 0.55 ± 0.05 h −1 which compared favorably with growth rate obtained in submerged culture of 1.28 ± 0.02 h −1 at 29 °C. The significance of the results is that microbial contamination in food processing plants can be assessed rapidly (<4 h) by measuring growth rate on piezoelectric-excited millimeter-sized glass cantilevers. Further, we compare its performance with QCM and find that PEMC is 1000 times more sensitive and 10 times quicker in establishing growth of bacteria.

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Web of Science research areas
Chemistry, Analytical
Electrochemistry
Instruments & Instrumentation
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