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Rapid, label-free genetic detection of enteropathogens in stool without genetic isolation or amplification
Journal article   Open access   Peer reviewed

Rapid, label-free genetic detection of enteropathogens in stool without genetic isolation or amplification

Song Han, Mehmet C. Soylu, Ceyhun E. Kirimli, Wei Wu, Bhaswati Sen, Suresh G. Joshi, Christopher L. Emery, Giang Au, Xiaomin Niu, Richard Hamilton, …
Biosensors & bioelectronics, v 130, pp 73-80
01 Apr 2019
DOI: https://doi.org/10.1016/j.bios.2019.01.025
PMID: 30731348
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1016/j.bios.2019.01.025View
Accepted (AM)Maybe Open Access (Publisher Bronze),  Open

Abstract

Amplification-free In situ Isolation-free Label-free Piezoelectric plate sensor Stool bacteria genetic detection
Current genetic detection methods require gene isolation, gene amplification and detection with a fluorescent-tagged probe. They typically require sophisticated equipment and expensive fluorescent probes, rendering them not widely available for rapid acute infection diagnoses at the point of care to ensure timely treatment of the diseases. Here we report a rapid genetic detection method that can detect the bacterial gene directly from patient stools using a piezoelectric plate sensor (PEPS) in conjunction with a continuous flow system with two temperature zones. With stools spiked with sodium dodecyl sulfate (SDS) in situ bacteria lysing and DNA denaturation occurred in the high-temperature zone whereas in situ specific detection of the denatured DNA by the PEPS occurred in the lower-temperature zone. The outcome was a rapid genetic detection method that directly detected bacterial genes from stool in < 40 min without the need of gene isolation, gene amplification, or expensive fluorescent tag but with polymerase chain reaction (PCR) sensitivity. In 40 blinded patient stools, it detected the toxin B gene of Clostridium difficile with 95% sensitivity and 95% specificity. The all-electrical, label-free nature of the detection further supports its potential as a low-cost genetic test that can be used at the point of care. •Piezoelectric sensor contains a flow system that lyses bacteria, exposes and de-hybridizes the genetic signature of the bacteria.•The genetic signature of bacteria is detected in situ in stool without gene isolation or amplification.•The detection is label-free with 150 CFU/ml sensitivity and in 40 min.•Toxin B gene of Clostridium difficile was detected in 40 patient stools with 95% sensitivity and 95% specificity.

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14 citations in Web of Science
17 citations in Scopus

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UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

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Web of Science research areas
Biophysics
Biotechnology & Applied Microbiology
Chemistry, Analytical
Electrochemistry
Nanoscience & Nanotechnology
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