Journal article
Rapid simultaneous assay of serum estrone, estradiol, and estriol in pregnant women using methyl ether acetate derivatives by capillary gas chromatography and electron-impact mass spectrometry
Journal of chromatography. Biomedical applications, v 272(2), pp 233-241
01 Jan 1983
PMID: 6220022
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A simple gas chromatographic—mass spectrometric method capable of measuring estrone, estradiol, and estriol simultaneously with a sensitivity close to that of radioimmunoassay has been developed. The estrogens in serum were extracted with diethyl ether, and internal standards (3-O-C
2H
3-estrone, 3-O-C
2H
3-estradiol, and 3-O-C
2H
3-estriol) were added, followed by converting to methyl ether compounds with an extractive alkylation procedure. The methyl ethers were then acetylated. Analyses were performed using a SP-2250 capillary column gas chromatograph coupled with an electron-impact mass spectrometer.
The estrogen methyl ether acetate derivatives were more stable chemically and gave less fragmentation upon electron impact than the conventional trimethylsilyl derivatives. The use of selected ion monitoring of molecular ions and that of the corresponding internal standards (M + 3) provides a sensitivity down to 10 pg for estrone and estradiol and to 200 pg for estriol. The time required for the preparation of multiple samples is within 4 hours.
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Details
- Title
- Rapid simultaneous assay of serum estrone, estradiol, and estriol in pregnant women using methyl ether acetate derivatives by capillary gas chromatography and electron-impact mass spectrometry
- Creators
- Kou-Yi TserngRobert K. DanishJuliana S. Bendt
- Publication Details
- Journal of chromatography. Biomedical applications, v 272(2), pp 233-241
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:A1983QE07100002
- Scopus ID
- 2-s2.0-0020692051
- Other Identifier
- 991021463580104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical