Journal article
Rapid visual detection of phytase gene in genetically modified maize using loop-mediated isothermal amplification method
Food chemistry, v 156, pp 184-189
01 Aug 2014
PMID: 24629956
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Transgenic maize plant expressing high phytase activity has been reported and approved by Chinese government in 2009. Here, we report a highly specific loop-mediated isothermal amplification (LAMP) method to detect the phytase gene in the GMO maize. The LAMP reaction takes less than 20 min and the amplification is visible without gel electrophoresis. The detection sensitivity of the LAMP method is about 30 copies of phytase genomic DNA, which is 33.3 times greater than the conventional PCR method with gel electrophoresis. The quantitative detection results showed that the LAMP method has a good linear correlation between the DNA copy number and the associated Tt values over a large dynamic range of template concentration from 6 x 10(1) to 6 x 107 copies, with a quantification limit of 60 copies. Therefore, the LAMP method is visual, faster, and more sensitive, and does not need special equipment compared to traditional PCR technique, which is very useful for field tests and fast screening of GMO feeds. (C) 2014 Elsevier Ltd. All rights reserved.
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Details
- Title
- Rapid visual detection of phytase gene in genetically modified maize using loop-mediated isothermal amplification method
- Creators
- Xin Huang - Chinese Academy of Inspection and QuarantineLili Chen - Chinese Academy of Inspection and QuarantineJiangmin Xu - Chinese Academy of Inspection and QuarantineHai-Feng Ji - Drexel UniversityShuifang Zhu - Chinese Academy of Inspection and QuarantineHongjun Chen - Chinese Academy of Inspection and Quarantine
- Publication Details
- Food chemistry, v 156, pp 184-189
- Publisher
- Elsevier
- Number of pages
- 6
- Grant note
- 2013ZX08012-001 / Important National Science & Technology Specific Projects
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000334138300028
- Scopus ID
- 2-s2.0-84896706545
- Other Identifier
- 991019330800104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Applied
- Food Science & Technology
- Nutrition & Dietetics