Journal article
Rates of reduced cytochrome c-ferricyanide binding and electron transfer
Biochimica et biophysica acta. Bioenergetics, v 548(2), pp 417-426
1979
PMID: 228712
Abstract
The oxidation of reduced cytochrome
c by ferricyanide has been studied over a wide range of ferricyanide concentrations using a continuous-flow apparatus. The formation of a ferrocytochrome
c-ferricyanide complex has been demonstrated and the binding and electron transfer processes separated to give both the oxidation electron transfer rate and the binding rate parameters. The electron transfer rate has been found to be 1.86 · 10
3 s
−1 in H
2O buffer and 1.36 · 10
3 s
−1 in
2H
2O demonstrating that a deuterium isotope effect of similar magnitude (
R = 1.37) to that found in the cytochrome reactions in photosynthetic bacteria [18] is also found in the reaction studied here. The binding association rate parameters also show a similar deuterium isotope effect suggesting that water rotation may be involved in both the binding of ferricyanide to reduced cytochrome
c and the subsequent oxidation electron transfer.
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Details
- Title
- Rates of reduced cytochrome c-ferricyanide binding and electron transfer
- Creators
- James A. Mccray - Drexel UniversityToru Kihara - University of Pennsylvania
- Publication Details
- Biochimica et biophysica acta. Bioenergetics, v 548(2), pp 417-426
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1979HV51500019
- Scopus ID
- 2-s2.0-0018785112
- Other Identifier
- 991019173895204721