Journal article
Real-time and label-free detection of cellular response to signaling mediated by distinct subclasses of epidermal growth factor receptors
Analytical chemistry (Washington), v 83(8), pp 3141-3146
15 Apr 2011
PMID: 21438528
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Epidermal growth factor receptors (EGFRs) have often shown two distinct binding affinities for epidermal growth factor. It is the high-affinity EGFR that is predominantly responsible for mediating the cell signaling that plays an indispensable role in cell growth, proliferation, motility, and differentiation. We applied the quartz crystal microbalance with dissipation monitoring (QCM-D) to track short-term cellular responses to EGFR signaling in human carcinoma A431 cells. Cellular responses to high- and low-affinity EGFR signaling were detected individually as well as simultaneously based on changes in mass and viscoelasticity of cells. These responses are associated with EGF-induced biological processes including the cytoskeleton remodeling and calcium influx. QCM-D provides a label-free sensor technology that can be exploited to investigate the role of high-affinity EGFR in cancer development and cancer prognosis.
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Details
- Title
- Real-time and label-free detection of cellular response to signaling mediated by distinct subclasses of epidermal growth factor receptors
- Creators
- Jennifer Y Chen - Chemistry Department, Drexel University, 3141 Chestnut Street, Philadelphia, Pennsylvania 19104, USAMinghong LiLynn S PennJun Xi
- Publication Details
- Analytical chemistry (Washington), v 83(8), pp 3141-3146
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000289223700042
- Scopus ID
- 2-s2.0-79954540536
- Other Identifier
- 991014877781904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical