Journal article
Real-time, in situ DNA hybridization detection with attomolar sensitivity without amplification using (pb(Mg1/3Nb2/3)O3)0.65–(PbTiO3)0.35 piezoelectric plate sensors
Biosensors & bioelectronics, v 43(1), pp 391-399
15 May 2013
PMID: 23356996
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In this paper we have investigated real-time, in situ DNA hybridization detection using piezoelectric plate sensors (PEPSs) consisting of a highly piezoelectric lead magnesium niobate-lead titanate (PMN-PT) layer 8μm in thickness thinly coated with Cr/Au electrodes and electrically insulated with 3-mercaptopropyltrimethoxysilane (MPS) encapsulation. With probe complementary DNA (cDNA) immobilized on the PEPS surface and by monitoring the first longitudinal extension mode (LEM) resonance frequency shift of the PEPS we detected hybridization of the target DNA (tDNA) to the probe cDNA on the PEPS surface in real time at concentration 1.6×10–18M with a signal to noise ratio of 8 without isolation and amplification at room temperature in 30min in phosphate buffered saline (PBS) solution. The detection was validated in situ by two different methods: (1) the detection of fluorescently labeled microspheres coated with reporter cDNA complementary to the tDNA but different from the probe cDNA; (2) fluorescent visualization.
► Piezoelectric plate sensor (PEPS) detects DNA in situ at 10−18M in 30min. ► PEPS detection is without isolation, concentration, and amplification. ► Attomolar sensitivity is validated in situ by reporter microspheres detection. ► Attomolar sensitivity is also validated by reporter microspheres visualization.
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Details
- Title
- Real-time, in situ DNA hybridization detection with attomolar sensitivity without amplification using (pb(Mg1/3Nb2/3)O3)0.65–(PbTiO3)0.35 piezoelectric plate sensors
- Creators
- Wei Wu - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USACeyhun E Kirimli - School of Biomedical Engineering, Science and health Systems, Drexel University, Philadelphia, PA 19104, USAWei-Heng Shih - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USAWan Y Shih - School of Biomedical Engineering, Science and health Systems, Drexel University, Philadelphia, PA 19104, USA
- Publication Details
- Biosensors & bioelectronics, v 43(1), pp 391-399
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
- Web of Science ID
- WOS:000316525300064
- Scopus ID
- 2-s2.0-84873809758
- Other Identifier
- 991014877719404721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology