Journal article
Real-time monitoring of adhesion and aggregation of platelets using thickness shear mode (TSM) sensor
Biosensors & bioelectronics, v 23(4), pp 575-582
2007
PMID: 17913487
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Hemostasis is required to maintain vascular system integrity, but thrombosis, formation of a clot in a blood vessel, is one of the largest causes of morbidity and mortality in the industrialized world. Novel clinical and research tools for characterizing the hemostatic system are of continued interest, and the object of this research is to test the hypothesis that clinically relevant platelet function can be monitored using an electromechanical sensor. A piezoelectric thickness shear mode (TSM) biosensor coated with collagen-I fibers to promote platelet activation and adhesion was developed and tested for sensitivity to detect these primary events. Magnitude and frequency response of the sensor were monitored under static conditions at 37
°C, using platelet-rich plasma (PRP), and PRP with adenosine diphosphate (ADP), a clinical aggregation inhibitor (abciximab), or a collagen binding inhibitor. Sensors loaded with PRP exhibited a 3-stage response; no significant change in response for the first 20
min (Stage-1), followed by a larger drop in response (Stage-2) and subsequently, response gradually increased (Stage-3). Exogenous ADP stimulated an immediate Stage-2 response, while abciximab delayed and reduced the magnitude change of Stage-2. In the presence of collagen inhibitor, Stage-2 response was similar to that of control but was delayed by an additional 20
min. The obtained results, supported by epifluorescence and complementary SEM studies, demonstrated the selective sensitivity of TSM electromechanical biosensors to monitor platelet function and inhibition, particularly aggregation.
Metrics
Details
- Title
- Real-time monitoring of adhesion and aggregation of platelets using thickness shear mode (TSM) sensor
- Creators
- E Ergezen - School of Biomedical Engineering, Science and Health Systems, Drexel University, United StatesM Appel - Mechanical Engineering & Mechanics, Drexel University, United StatesP Shah - School of Biomedical Engineering, Science and Health Systems, Drexel University, United StatesJ.Y Kresh - Department of Cardiothoracic Surgery, Drexel University College of Medicine, United StatesR.M Lec - School of Biomedical Engineering, Science and Health Systems, Drexel University, United StatesD.M Wootton - Mechanical Engineering & Mechanics, Drexel University, United States
- Publication Details
- Biosensors & bioelectronics, v 23(4), pp 575-582
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; [Retired Faculty]
- Web of Science ID
- WOS:000251120200019
- Scopus ID
- 2-s2.0-35348847090
- Other Identifier
- 991014878441504721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology