Journal article
Reassembly of C- myc and relaxation of C- fos nucleosomes during differentiation of human leukemic (HL-60) cells
Biochemical and biophysical research communications, v 141(1), pp 213-221
1986
PMID: 3541926
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human promyelocytic leukemic (HL-60) cells (1) have amplified c-
myc protooncogene sequences which lead to an elevated level of c-
myc gene expression (2,3). Induction of HL-60 cells by phorbol esters to undergo monocytic differentiation (4) results in the suppression of c-
myc (5), but the activation of c-
fos gene transcription (6). Chromatin structures of c-
myc and c-
fos were compared by measuring their sequences in nucleosome-associated DNA fragments. These nucleosomal particles were released from chromatin by micrococcal nuclease digestion and subsequently analyzed with two dimensional gel electrophoresis. C-
myc related sequences were detected in nucleosomal DNA fragments of differentiated cells only, while the c-
fos related sequences were found in nucleosomal DNAs of noninduced HL-60 cells. Since the enzyme preferentially digests relaxed DNAs, these results suggest that nucleosomal subunits of c-
myc and c-
fos chromatin are relaxed during the state of active transcription, and reassembled once their transcription is repressed.
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Details
- Title
- Reassembly of C- myc and relaxation of C- fos nucleosomes during differentiation of human leukemic (HL-60) cells
- Creators
- Robin H. Chou - Department of Anatomy Hahnemann University School of Medicine Philadelphia, Pa 19102, USAThelma A. Chen - Department of Anatomy Hahnemann University School of Medicine Philadelphia, Pa 19102, USAJudy R. Churchill - Department of Anatomy Hahnemann University School of Medicine Philadelphia, Pa 19102, USAScott W. Thompson - Department of Anatomy Hahnemann University School of Medicine Philadelphia, Pa 19102, USAKoulin L. Chou - Department of Anatomy Hahnemann University School of Medicine Philadelphia, Pa 19102, USA
- Publication Details
- Biochemical and biophysical research communications, v 141(1), pp 213-221
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Intensive Medical Sciences (IMS)
- Web of Science ID
- WOS:A1986F209000031
- Scopus ID
- 2-s2.0-0022916125
- Other Identifier
- 991019184041804721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics