Journal article
Recombinant-protein production in insect cells utilizing a hollow-fibre bioreactor
Biotechnology and applied biochemistry, v 45(Pt 3)
Nov 2006
PMID: 16872272
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Here, we demonstrate for the first time that the hollow-fibre bioreactor is an excellent tool for the production of Drosophila-expressed recombinant proteins. Using the example of the soluble extracellular portion of the human IL-5 (interleukin 5) receptor alpha expression in S2 (Schneider's Drosophila melanogaster cell line 2) cells, we found that it is possible to produce multi-milligram amounts of functional recombinant protein continuously for several months on a laboratory scale with minimal maintenance requirements. The insect cells grow to high density and express concentrated functional recombinant protein in a small volume, simplifying and economizing downstream purification.
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Details
- Title
- Recombinant-protein production in insect cells utilizing a hollow-fibre bioreactor
- Creators
- Sabine C Baxter - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 11102 New College Building, 245 N. 15th Street, Philadelphia, PA 19102, USA. scb28@drexel.eduDominick PanarelloSandya AjithMadhushree BhattacharyaIrwin M Chaiken
- Publication Details
- Biotechnology and applied biochemistry, v 45(Pt 3)
- Publisher
- United States
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000241975100007
- Scopus ID
- 2-s2.0-33750620312
- Other Identifier
- 991014878517304721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology