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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Reconstituting the 4-Strand DNA Strand Exchange
Methods in enzymology, v 600
2018
PMID: 29458763
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Proteins of the Rad51 family play a key role in homologous recombination by carrying out DNA strand exchange. Here, we present the methodology and the protocols for the 4-strand exchange between gapped circular DNA and homologous linear duplex DNA promoted by human Rad51 and Escherichia coli RecA orthologs. This reaction includes formation of joint molecules and their extension by branch migration in a polar manner. The presented methodology may be used for reconstitution of the medial-to-late stages of homologous recombination in vitro as well as for investigation of the mechanisms of branch migration by helicase-like proteins, e.g., Rad54, BLM, or RecQ1.
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Details
- Title
- Reconstituting the 4-Strand DNA Strand Exchange
- Creators
- Olga M Mazina - Drexel UniversityAlexander V Mazin - Drexel University
- Publication Details
- Methods in enzymology, v 600
- Publisher
- Elsevier
- Grant note
- P30 CA056036 / NCI NIH HHS R01 CA188347 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000452357600012
- Scopus ID
- 2-s2.0-85040585689
- Other Identifier
- 9780128144299; 0128144297; 991019168694604721
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- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology