Journal article
Reconstitution of Neuronal Motor Traffic on Septin-Associated Microtubules
Methods in molecular biology (Clifton, N.J.), v 2794, pp 79-94
2024
PMID: 38630222
Abstract
Reconstitution of intracellular transport in cell-free in vitro assays enables the understanding and dissection of the molecular mechanisms that underlie membrane traffic. Using total internal reflection fluorescence (TIRF) microscopy and microtubules, which are immobilized to a functionalized glass surface, the kinetic properties of single kinesin molecules can be imaged and analyzed in the presence or absence of microtubule-associated proteins. Here, we describe methods for the in vitro reconstitution of the motility of the neuronal kinesin motor KIF1A on microtubules associated with heteromeric septin (SEPT2/6/7) complexes. This method can be adapted for various neuronal septin complexes and kinesin motors, leading to new insights into the spatial regulation of neuronal membrane traffic by microtubule-associated septins.
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Details
- Title
- Reconstitution of Neuronal Motor Traffic on Septin-Associated Microtubules
- Creators
- Yani Suber - Drexel UniversityElias T Spiliotis - Drexel University
- Publication Details
- Methods in molecular biology (Clifton, N.J.), v 2794, pp 79-94
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Scopus ID
- 2-s2.0-85190902517
- Other Identifier
- 9781071638101; 1071638106; 991021868101104721