Journal article
Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration
Human molecular genetics, v 14(21), pp 3113-3128
01 Nov 2005
PMID: 16203747
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
LIS1 is mutated in the human neuronal migration defect lissencephaly and along with NDEL1 (formerly NUDEL) participates in the regulation of cytoplasmic dynein function during neuronal development. Targeted disruption of Ndel1 suggested that NDEL1 could have other molecular targets that regulate microtubule organization for proper neuronal migration. To further understanding the molecular mechanism of LIS1 and lissencephaly, we identified the katanin p60 microtubule-severing protein as an additional molecular target of NDEL1. We demonstrate that phosphorylation of NDEL1 by Cdk5 facilitates interaction between NDEL1 and p60, suggesting that P-NDEL1 regulates the distribution of katanin p60. Abnormal accumulation of p60 in nucleus of Ndel1 null mutants supports an essential role of NDEL1 in p60 regulation. Complete loss of NDEL1 or expression of dominant negative mutants of p60 in migrating neurons results in defective migration and elongation of nuclear-centrosomal distance. Our results suggest that NDEL1 is essential for mitotic cell division and neuronal migration not only via regulation of cytoplasmic dynein function but also by modulation of katanin p60 localization and function.
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Details
- Title
- Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration
- Creators
- Kazuhito Toyo-Oka - The University of OsakaShinji Sasaki - Keio UniversityYoshihisa Yano - Osaka City UniversityDaisuke Mori - Osaka City UniversityTakuya Kobayashi - The University of TokyoYoko Y. Toyoshima - The University of TokyoSuzumi M. Tokuoka - The University of TokyoSatoshi Ishii - The University of TokyoTakao Shimizu - The University of TokyoMasami Muramatsu - Saitama Medical UniversityNoriko Hiraiwa - RIKEN BioResource Research CenterAtsushi Yoshiki - RIKEN BioResource Research CenterAnthony Wynshaw-Boris - University of California San DiegoShinji Hirotsune - Saitama Medical University
- Publication Details
- Human molecular genetics, v 14(21), pp 3113-3128
- Publisher
- Oxford University Press
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000232748800001
- Scopus ID
- 2-s2.0-27744560966
- Other Identifier
- 991020100076004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Genetics & Heredity