Journal article
Regeneration of bombyx mori silk by electrospinning: A comparative study of the biocompatibility of natural and synthetic polymers for tissue engineering applications
Journal of biobased materials and bioenergy, v 1(2), pp 274-281
01 Aug 2007
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The nanofibers produced from the regenerated Bombyx mori silk are the ideal candidate for tissue engineering scaffolds because of the advantage of having high surface area to volume ratio. We carried out the comparison of mechanical and biological properties between natural and synthetic polymeric nanofibers fabricated by co-electrospinning procedure. Various blends of regenerated silk with 1% (w/w) Type I collagen and 1% (w/w) polylactic-co-glycolic acid (PLAGA) were obtained using Hexafluoro isopropanol (HFIP) as a solvent. The parameters for electrospinning were optimized for fabricating the scaffolds with relatively uniform fiber diameters (approximately 400 nm). The morphology of electrospun fibers was characterized by environmental scanning electron microscopy (ESEM). The mechanical properties were determined via microtensile testing and cell-scaffolds interaction study was conducted using primary fetal bovine osteoblasts cells. Our results suggested that elasticity of silk nanofibers was altered with the incorporation of collagen and PLAGA. The elongation of silk nanofibers changed from 5.54 +/- 0.25% to 6.26 +/- 0.63% for silk-collagen nanofibers and to 9.86 +/- 0.22% for silk-PLAGA nanofibers. Silk nanofibers had comparable biocompatibility to collagen nanofibers. This proves that silk can be used instead of collagen in fabricating tissue engineering scaffolds. Consequently Silk can be employed as a cheaper alternative for collagen to fabricate tissue engineering scaffold, with adequate mechanical properties, and with equal capability of growing and differentiating osteoblast cells.
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Details
- Title
- Regeneration of bombyx mori silk by electrospinning: A comparative study of the biocompatibility of natural and synthetic polymers for tissue engineering applications
- Creators
- Milind Gandhi - Drexel Univ, Sch Biomed Engn, Philadelphia, PA 19104 USAHeejae Yang - Drexel Univ, Sch Biomed Engn, Philadelphia, PA 19104 USALauren Shor - Drexel Univ, Dept Mech Engn & Mech, Philadelphia, PA 19104 USAFrank Ko - Drexel University
- Publication Details
- Journal of biobased materials and bioenergy, v 1(2), pp 274-281
- Publisher
- Amer Scientific Publishers
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000253339900012
- Other Identifier
- 991019168233704721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Applied
- Energy & Fuels
- Materials Science, Biomaterials