Journal article
Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor
Nature methods, v 3(6), pp 455-460
01 Jun 2006
PMID: 16721379
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Multiple nuclear transcription factors including E-26-like protein 1 (Elk-1) have been found in neuronal dendrites, yet the functional significance of such localization has not yet been explained. Here we use a focal transfection procedure, 'phototransfection', to introduce Elk1 mRNA into specific regions of live, intact primary rat neurons. Introduction and translation of Elk1 mRNA in dendrites produced cell death, whereas introduction and translation of Elk1 mRNA in cell bodies did not produce cell death. Elk-1 translated in dendrites was transported to the nucleus, and cell death depended upon transcription, supporting the dendritic imprinting hypothesis and highlighting the importance of the dendritic environment on protein function. Our demonstration of the utility of phototransfection for spatially controlled introduction of mRNAs opens the broader opportunity to use this method to introduce selected quantities of small molecules into discrete regions of live cells to assess their biological functions.
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Details
- Title
- Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor
- Creators
- Lindy E Barrett - University of PennsylvaniaJai-Yoon SulHajime Takano - University of PennsylvaniaElisabeth J Van Bockstaele - Thomas Jefferson UniversityPhilip G HaydonJames H Eberwine - University of Pennsylvania
- Publication Details
- Nature methods, v 3(6), pp 455-460
- Publisher
- Springer Nature
- Grant note
- MH74169 / NIMH NIH HHS MH58561 / NIMH NIH HHS R37NS037585 / NINDS NIH HHS DA015395 / NIDA NIH HHS R01NS043142 / NINDS NIH HHS AG9900 / NIA NIH HHS P20MH071705 / NIMH NIH HHS P30NS047321 / NINDS NIH HHS DA09082 / NIDA NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000238124700014
- Scopus ID
- 2-s2.0-33744505179
- Other Identifier
- 991021903297704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods