Journal article
Regional and Temporal Alterations in DNA Fragmentation Factor (DFF)‐Like Proteins Following Experimental Brain Trauma in the Rat
Journal of neurochemistry, v 73(4), pp 1650-1659
Oct 1999
PMID: 10501212
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Abstract
: DNA fragmentation, an early event in neuronal death
following traumatic brain injury, may be triggered by the 40‐kDa subunit of
DNA fragmentation factor (DFF40). DFF40 is typically bound to the 45‐kDa
subunit of DFF (DFF45), and activation of DFF40 may occur as a result of
caspase‐3‐mediated cleavage of DFF45 into 30‐and 11‐kDa fragments. In this
study, the intracellular distribution of DFF45 and DFF40 was examined
following lateral fluid percussion brain injury of moderate severity (2.4‐2.7
atm) in male Sprague‐Dawley rats. In the cytosolic fraction (S1) of the
injured cortex at 2 and 24 h postinjury, significant decreases in the
intensities of DFF45‐like proteins at 45‐ and 32‐kDa bands and a concomitant
increase in the 11‐kDa bands were observed (p <0.05 vs. uninjured
controls). A significant decrease in the intensities of the 32‐kDa band in the
nuclear (P1) fraction of the injured cortex was observed at 30 min and 2 h
postinjury (p < 0.01). Concomitantly, a decrease in DFF40 was
observed in the cortical S1 fraction at 2 and 24 h (p < 0.05) and
in the P1 fraction at 30 min and 2 h postinjury (p < 0.01). In the
hippocampus, DFF45 decreased at 30 min in the P1 and 2 h in the S1 fraction
(p <0.05) and recovered by 24 h postinjury, whereas DFF40 was
significantly decreased in the S1 and increased in the P1 fraction at both 2
and 24 h (p < 0.01), which indicated a translocation of DFF40 from cytosol to nucleus. These data are the first to demonstrate that changes in DFF proteins occur after brain trauma and suggest that these changes may play a role in apoptotic cell death via caspase‐3‐DFF45/DFF40‐DNA cleavage observed following traumatic brain injury.
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Details
- Title
- Regional and Temporal Alterations in DNA Fragmentation Factor (DFF)‐Like Proteins Following Experimental Brain Trauma in the Rat
- Creators
- Chen ZhangRamesh RaghupathiKathryn E SaatmanMichelle C LaPlacaTracy K McIntosh
- Publication Details
- Journal of neurochemistry, v 73(4), pp 1650-1659
- Publisher
- Blackwell Science Ltd; Oxford UK
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000082616200037
- Scopus ID
- 2-s2.0-0032824031
- Other Identifier
- 991014877868404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences