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Regional biomechanical imaging of liver cancer cells
Journal article   Open access   Peer reviewed

Regional biomechanical imaging of liver cancer cells

Weiwei Pei, Jiayao Chen, Chao Wang, Suhao Qiu, Jianfeng Zeng, Mingyuan Gao, Bin Zhou, Dan Li, Michael S. Sacks, Lin Han, …
Journal of Cancer, v 10(19), pp 4481-4487
01 Jan 2019
PMID: 31528212
url
https://doi.org/10.7150/jca.32985View
Published, Version of Record (VoR)CC BY-NC V4.0 Open

Abstract

Life Sciences & Biomedicine Oncology Science & Technology
Liver cancer is one of the leading cancers, especially in developing countries. Understanding the biomechanical properties of the liver cancer cells can not only help to elucidate the mechanisms behind the cancer progression, but also provide important information for diagnosis and treatment. At the cellular level, we used well-established atomic force microscopy (AFM) techniques to characterize the heterogeneity of mechanical properties of two different types of human liver cancer cells and a normal liver cell line. Stiffness maps with a resolution of 128x128 were acquired for each cell. The distributions of the indentation moduli of the cells showed significant differences between cancerous cells and healthy controls. Significantly, the variability was even greater amongst different types of cancerous cells. Fitting of the histogram of the effective moduli using a normal distribution function showed the Bel7402 cells were stiffer than the normal cells while HepG2 cells were softer. Morphological analysis of the cell structures also showed a higher cytoskeleton content among the cancerous cells. Results provided a foundation for applying knowledge of cell stiffness heterogeneity to search for tissue-level, early-stage indicators of liver cancer.

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Oncology
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