Journal article
Regulating Mechanotransduction in Three Dimensions using Sub-Cellular Scale, Crosslinkable Fibers of Controlled Diameter, Stiffness, and Alignment
Advanced functional materials, v 29(18), pp 1-11
02 May 2019
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The extracellular matrix (ECM) is a complex 3D framework of macromolecules, which regulate cell bioactivity via chemical and physical properties. The ECM's physical properties, including stiffness and physical constraints to cell shape, regulate actomyosin cytoskeleton contractions, which induce signaling cascades influencing gene expression and cell fate. Engineering such bioactivity, a.k.a., mechanotransduction, has been mainly achieved by 2D platforms such as micropatterns. These platforms cause cytoskeletal contractions with apico-basal polarity and can induce mechanotransduction that is unnatural to most cells in native ECMs. An effective method to engineer mechanotransduction in 3D is needed. This work creates FiberGel, a 3D artificial ECM comprised of sub-cellular scale fibers. These microfibers can crosslink into defined microstructures with the fibers' diameter, stiffness, and alignment independently tuned. Most importantly, cells are blended amongst the fibers prior to crosslinking, leading to homogeneously cellularized scaffolds. Studies using mesenchymal stem cells showed that the microfibers' diameter, stiffness, and alignment regulate 3D cell shape and the nuclei translocation of transcriptional coactivators YAP/TAZ (yes-associated protein/transcriptional coactivator), which enables the control of cell differentiation and tissue formation. A novel technology based on repeated stretching and folding is created to synthesize FiberGel. This 3D platform can significantly contribute to mechanotransduction research and applications.
Metrics
Details
- Title
- Regulating Mechanotransduction in Three Dimensions using Sub-Cellular Scale, Crosslinkable Fibers of Controlled Diameter, Stiffness, and Alignment
- Creators
- Mingkun Wang - Drexel UniversityChunxiao Cui - Drexel UniversityMazen Mohamed Ibrahim - Helwan UniversityBiao Han - Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USAQing Li - Drexel UniversityMaurizio Pacifici - University of PennsylvaniaJohn Todd R. Lawrence - University of PennsylvaniaLin Han - Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USALi-Hsin Han - Drexel Univ, Dept Mech Engn & Mech, 3141 Chestnut St, Philadelphia, PA 19104 USA
- Publication Details
- Advanced functional materials, v 29(18), pp 1-11
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- Drexel University CBET-1605604 / National Science Foundation; National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000471330500014
- Scopus ID
- 2-s2.0-85063382785
- Other Identifier
- 991019168399104721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied
- Physics, Condensed Matter