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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Regulation of Axon Guidance and Extension by 3-Dimensional Constraints
Biomaterials, v 28(23), pp 3398-3407
14 Apr 2007
PMID: 17467794
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Axons
in vivo
are guided by molecular signals acting as attractants and repellents, and possibly by physical constraints encountered in the extracellular environment. We analyzed the ability of primary sensory axons to extend and undergo guidance in 3-D environments generated using photolithography. Confinement of neurons in fully enclosed square chambers decreased the percentage of neurons establishing axons as a function of chamber width. However, the ability to extend an axon in one or more directions allowed axons to form and extend similarly to those on 2-D substrata. Live imaging of growth cones interacting with the walls of chambers or corridors revealed that growth cones respond to contact with a 3-D constraint by decreasing surface area, and circumvent constraints by repeated sampling of the constraint until an unobstructed path is encountered. Analysis of the ability of axons to turn around corners in corridors revealed that the angle of the corner and corridor width determined the frequency of turning. Finally, we show that the length of axons can be controlled through the use of 3-D constraints. These data demonstrate that 3-D constraints can be used to guide axons, and control the extent of axon formation and the length of axons.
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Details
- Title
- Regulation of Axon Guidance and Extension by 3-Dimensional Constraints
- Creators
- Herbert Francisco - Drexel UniversityBenjamin B. Yellen - Duke UniversityDerek S. Halverson - Drexel UniversityGary Friedman - Drexel UniversityGianluca Gallo - Drexel University
- Publication Details
- Biomaterials, v 28(23), pp 3398-3407
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering
- Web of Science ID
- WOS:000247362700004
- Scopus ID
- 2-s2.0-34248178242
- Other Identifier
- 991019196805404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials