Journal article
Regulation of Lipid Biosynthesis in Saccharomyces cerevisiae by Fumonisin B1
The Journal of biological chemistry, v 270(22), pp 13171-13178
02 Jun 1995
PMID: 7768913
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Abstract
The regulation of lipid biosynthesis in the yeast Saccharomyces cerevisiae by fumonisin B1 was examined. Fumonisin B1 inhibited the growth of yeast cells. Cells supplemented with fumonisin B1 accumulated free sphinganine and phytosphingosine in a dose-dependent manner. The cellular concentration of ceramide was reduced in fumonisin B1-supplemented cells. Ceramide synthase activity was found in yeast cell membranes and was inhibited by fumonisin B1. Fumonisin B1 inhibited the synthesis of the inositol-containing sphingo-lipids inositol phosphorylceramide, mannosylinositol phosphorylceramide, and mannosyldiinositol phosphorylceramide. Fumonisin B1 also caused a decrease in the synthesis of the major phospholipids synthesized via the CDP-diacylglycerol-dependent pathway and the synthesis of neutral lipids. The effects of fumonisin B1 and sphingoid bases on the activities of enzymes in the pathways leading to the synthesis of sphingolipids, phospholipids, and neutral lipids were also examined. Other than ceramide synthase, fumonisin B1 did not affect the activities of any of the enzymes examined. However, sphinganine and phytosphingosine inhibited the activities of inositol phosphorylceramide synthase, phosphatidylserine synthase, and phosphatidate phosphatase. These are key enzymes responsible for the synthesis of lipids in yeast. The data reported here indicated that the biosynthesis of sphingolipids, phospholipids and neutral lipids was coordinately regulated by fumonisin B1 through the regulation of lipid biosynthetic enzymes by sphingoid bases.
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Details
- Title
- Regulation of Lipid Biosynthesis in Saccharomyces cerevisiae by Fumonisin B1
- Creators
- Wen-I Wu - Rutgers, The State University of New JerseyVirginia M. McDonough - Rutgers, The State University of New JerseyJoseph T. Nickels - Rutgers, The State University of New JerseyJesang Ko - University of Rhode IslandAnthony S. Fischl - University of Rhode IslandTeresa R. Vales - Emory UniversityAlfred H. Merrill - Emory UniversityGeorge M. Carman - Rutgers, The State University of New Jersey
- Publication Details
- The Journal of biological chemistry, v 270(22), pp 13171-13178
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1995RB43900036
- Scopus ID
- 2-s2.0-0029012233
- Other Identifier
- 991021229907004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology