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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Regulation of adipogenesis by cytoskeleton remodelling is facilitated by acetyltransferase MEC-17-dependent acetylation of α-tubulin
Biochemical journal, v 449(3), pp 605-612
01 Feb 2013
PMID: 23126280
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cytoskeleton remodelling is a prerequisite step for the morphological transition from preadipocytes to mature adipocytes. Although microtubules play a pivotal role in organizing cellular structure, regulation of microtubule dynamics during adipogenesis remains unclear. In the present paper we show that acetylation of
α
-tubulin is up-regulated during adipogenesis, and adipocyte development is dependent on
α
-tubulin acetylation, as expression of an acetylation-resistant
α
-tubulin mutant significantly inhibits adipogenesis. Moreover, acetylation of
α
-tubulin is under the control of the acetyltransferase MEC-17 and deacetylases SIRT2 (Sirtuin 2) and HDAC6 (histone deacetylase 6). Adipocyte development is inhibited in MEC-17-knockdown cells, but enhanced in MEC-17-overexpressing cells. Finally, we show that katanin, a microtubule-severing protein with enhanced activity on acetylated
α
-tubulin, is actively involved in adipogenesis. We propose that co-ordinated up-regulation of
α
-tubulin acetylation initiates cytoskeleton remodelling by promoting
α
-tubulin severing by katanin which, in turn, allows expansion of lipid droplets and accelerates the morphological transition toward mature adipocytes.
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Details
- Title
- Regulation of adipogenesis by cytoskeleton remodelling is facilitated by acetyltransferase MEC-17-dependent acetylation of α-tubulin
- Creators
- Wulin Yang - Laboratory of Metabolic Medicine Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR) SingaporeXiangxiang Guo - Laboratory of Metabolic Medicine Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR) SingaporeShermaine Thein - Laboratory of Metabolic Medicine Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR) SingaporeFeng Xu - Singapore Institute for Clinical SciencesShigeki Sugii - Duke NUS Graduate Medical SchoolPeter W. Baas - Drexel UniversityGeorge K. Radda - Laboratory of Metabolic Medicine Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR) SingaporeWeiping Han - Singapore Bioimaging Consortium
- Publication Details
- Biochemical journal, v 449(3), pp 605-612
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000313776000004
- Scopus ID
- 2-s2.0-84872441219
- Other Identifier
- 991019167911404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology