Logo image
Back
Regulation of herpes simplex virus type 1 gene expression in nonpermissive murine resident peritoneal macrophages
Journal article   Peer reviewed

Regulation of herpes simplex virus type 1 gene expression in nonpermissive murine resident peritoneal macrophages

Linxian Wu, Page S. Morahan and Kathryn Leary
Journal of leukocyte biology, v 53(1), pp 61-65
Jan 1993
DOI: https://doi.org/10.1002/jlb.53.1.61
PMID: 8381150
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

herpesvirus mononuclear phagocyte nonpermissivity
The restriction of herpes simplex virus type 1 (HSV‐1) gene expression in nonpermissive murine resident peritoneal macrophages (ResPMO) was analyzed at the level of transcription. Using nuclear run‐on assays, we established that transcription of a representative gene from each HSV‐1 kinetic class was initiated in both ResPMO and permissive Vero cells. Using transient expression assays, we observed that the promoters of two early genes were activated by the immediate‐early protein ICP4 in ResPMO. However, the level of trans‐ activation of these promoters by another immediate‐early protein, ICPO, was markedly reduced in ResPMO. Furthermore, the synergistic trans‐activation normally observed between ICPs 4 and O in Vero cells was noticeably absent in ResPMO. The data indicate that the block in viral gene expression in ResPMO occurs after initiation of viral gene transcription and involves dysfunction of a viral immediate‐early regulatory protein, ICP0.

Metrics

8 Record Views
7 citations in Web of Science
7 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Web of Science research areas
Cell Biology
Hematology
Immunology
Logo image