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Journal article
Regulatory loop between the CsrA system and NhaR, a high salt/high pH regulator
PloS one, v 13(12), pp e0209554-e0209554
2018
PMID: 30589862
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In E. coli, under high pH/high salt conditions, a major Na+/H+ antiporter (NhaA) is activated to maintain an internal pH level. Its expression is induced by a specific regulator NhaR, which is also responsible for osmC and pgaA regulation. Here I report that the NhaR regulator affects the carbon storage regulatory Csr system. I found that the expression of all major components of the Csr system-CsrA regulator, CsrB and CsrC small RNAs, and the CsrB and CsrC stability were indirectly affected by nhaR mutation under stress conditions. Using a combination of experimental and in silico analyses, I concluded that the mechanism of regulation included direct and indirect activation of a two-component system (TCS) response regulator-UvrY. NhaR regulation involved interactions with the regulators H-NS and SdiA and was affected by a naturally occurring spontaneous IS5 insertion in the promoter region. A regulatory circuit was proposed and discussed.
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Details
- Title
- Regulatory loop between the CsrA system and NhaR, a high salt/high pH regulator
- Creators
- Jarosław E Król - University of Florida
- Publication Details
- PloS one, v 13(12), pp e0209554-e0209554
- Publisher
- Public LIbrary of Science (PLOS)
- Grant note
- R01 GM066794 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000454418200029
- Scopus ID
- 2-s2.0-85059239477
- Other Identifier
- 991019168575704721
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- Web of Science research areas
- Microbiology