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Repeated Reserpine Increases Striatal Dopamine Receptor and Guanine Nucleotide Binding Protein RNA
Journal article   Open access   Peer reviewed

Repeated Reserpine Increases Striatal Dopamine Receptor and Guanine Nucleotide Binding Protein RNA

Paul Butkerait and Eitan Friedman
Journal of neurochemistry, v 60(2), pp 566-571
Feb 1993
PMID: 8419537
url
https://doi.org/10.1111/j.1471-4159.1993.tb03186.xView
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

D1 and D2 dopamine receptor mRNA Guanine nucleotide binding protein mRNA Supersensitivity of striatal dopaminergic mechanism
: In the present study the effects of repeated administration of reserpine on striatal dopamine receptor and guanine nucleotide binding protein mRNAs were determined. Twenty‐four hours after seven consecutive daily injections of reserpine—a treatment that is known to produce functional sensitization of D1 and D2 dopamine receptors—the level of striatal D1 dopamine receptor mRNA was unchanged. However, the level of mRNA for the G protein Gsα was increased by 127%. After extended reserpine treatment for 14 days, levels of both striatal D1 DA receptor and Gsα mRNAs were elevated by 99 and 78%, respectively. Seven days of reserpine treatment also increased levels of mRNA of the striatal D2 dopamine receptor and of G proteins Gi2α and Goα by 200, 79, and 32%, respectively. After 14 days of reserpine treatment the level of striatal D2 dopamine receptor mRNA was increased by twofold. In contrast, levels of the mRNAs coding for the G proteins Gi2α and Goα were unchanged. These data suggest that dopamine receptors and their respective G proteins play important roles in the development of sensitization of striatal dopamine receptors during repeated reserpine treatment. Furthermore, the persistent increase in level of striatal Gsα mRNA suggests that this G protein is necessary to maintain supersensitivity of the striatal D1 dopamine receptor system following long‐term dopamine depletion.

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Biochemistry & Molecular Biology
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