Journal article
Replication Checkpoint Kinase Cds1 Regulates Recombinational Repair Protein Rad60
Molecular and cellular biology, v 23(16), pp 5939-5946
Aug 2003
PMID: 12897162
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Genome integrity is protected by Cds1 (Chk2), a checkpoint kinase that stabilizes arrested replication forks. How Cds1 accomplishes this task is unknown. We report that Cds1 interacts with Rad60, a protein required for recombinational repair in fission yeast. Cds1 activation triggers Rad60 phosphorylation and nuclear delocalization. A Rad60 mutant that inhibits regulation by Cds1 renders cells specifically sensitive to replication fork arrest. Genetic and biochemical studies indicate that Rad60 functions codependently with Smc5 and Smc6, subunits of an SMC (structural maintenance of chromosomes) complex required for recombinational repair. These studies indicate that regulation of Rad60 is an important part of the replication checkpoint response controlled by Cds1. We propose that control of Rad60 regulates recombination events at stalled forks.
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Details
- Title
- Replication Checkpoint Kinase Cds1 Regulates Recombinational Repair Protein Rad60
- Creators
- Michael N Boddy - Department of Molecular BiologyPaul Shanahan - Department of Molecular BiologyW Hayes McDonald - Department of Molecular BiologyAntonia Lopez-Girona - Department of Molecular BiologyEishi Noguchi - Department of Molecular BiologyJohn R Yates III - Department of Molecular BiologyPaul Russell - Department of Molecular Biology
- Publication Details
- Molecular and cellular biology, v 23(16), pp 5939-5946
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000184527100039
- Scopus ID
- 2-s2.0-0042131895
- Other Identifier
- 991014878105804721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology