Journal article
Replication competent HIV-1 viruses that express intragenomic microRNA reveal discrete RNA-interference mechanisms that affect viral replication
Cell & bioscience, v 1(1), 38
23 Nov 2011
PMID: 22112720
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background: It remains unclear whether retroviruses can encode and express an intragenomic microRNA (miRNA). Some have suggested that processing by the Drosha and Dicer enzymes might preclude the viability of a replicating retroviral RNA genome that contains a cis-embedded miRNA. To date, while many studies have shown that lentiviral vectors containing miRNAs can transduce mammalian cells and express the inserted miRNA efficiently, no study has examined the impact on the replication of a lentivirus such as HIV-1 after the deliberate intragenomic insertion of a bona fide miRNA.
Results: We have constructed several HIV-1 molecular clones, each containing a discrete cellular miRNA positioned in Nef. These retroviral genomes express the inserted miRNA and are generally replication competent in T-cells. The inserted intragenomic miRNA was observed to elicit two different consequences for HIV-1 replication. First, the expression of miRNAs with predicted target sequences in the HIV-1 genome was found to reduce viral replication. Second, in one case, where an inserted miRNA was unusually well-processed by Drosha, this processing event inhibited viral replication.
Conclusion: This is the first study to examine in detail the replication competence of HIV-1 genomes that express cis-embedded miRNAs. The results indicate that a replication competent retroviral genome is not precluded from encoding and expressing a viral miRNA.
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Details
- Title
- Replication competent HIV-1 viruses that express intragenomic microRNA reveal discrete RNA-interference mechanisms that affect viral replication
- Creators
- Zachary Klase - National Institute of Allergy and Infectious DiseasesLaurent Houzet - National Institute of Allergy and Infectious DiseasesKuan-Teh Jeang - NIAID, Mol Virol Sect, Mol Microbiol Lab, Bethesda, MD 20892 USA
- Publication Details
- Cell & bioscience, v 1(1), 38
- Publisher
- Springer Nature
- Number of pages
- 13
- Grant note
- NIAID; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) ZIAAI000547 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000307060600001
- Scopus ID
- 2-s2.0-84860494706
- Other Identifier
- 991021902600604721
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- Web of Science research areas
- Biochemistry & Molecular Biology