Journal article
Repression of Transcription and Interference with DNA Binding of TATA-Binding Protein by C-Terminal Alternatively Spliced p53
Experimental cell research, v 279(2)
2002
PMID: 12243750
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The protein encoded by C-terminal alternatively spliced p53 mRNA (p53as) has been shown previously to occur naturally in mouse cells and to bind sequence-specifically to DNA more efficiently than p53 (p53r, regular form). In the current study, p53as and p53r proteins ectopically expressed in p53-deficient cells each transactivated reporter plasmids containing p53 binding sites. However, p53as consistently was more efficient in transcriptional repression of promoters lacking p53 binding sites and in concentration-dependent repression of the
p21
WAF1/Cip-l/Sdi
promoter sequence. The p53as protein, like p53r, associated with TATA-binding protein (TBP), indicating that this interaction does not require the last 26 amino acids of p53. Consistent with its stronger repression effects, p53as interfered with TBP binding to a TATA-containing DNA sequence more efficiently than p53r protein. Taken together, these
in vitro and
in vivo results demonstrate a novel role in transcriptional repression for a naturally occurring C-terminal variant form of mouse p53 protein associated with differences in DNA binding properties and interference with transcription factor binding.
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Details
- Title
- Repression of Transcription and Interference with DNA Binding of TATA-Binding Protein by C-Terminal Alternatively Spliced p53
- Creators
- Hua HuangShinsuke KakuChad D KnightsByung S ParkJane CliffordMolly Kulesz-Martin
- Publication Details
- Experimental cell research, v 279(2)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000178224700008
- Scopus ID
- 2-s2.0-0036405612
- Other Identifier
- 991014877755104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology
- Oncology