Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone

Michelle H Theus; Jerome Ricard; Daniel J Liebl

doi:10.1002/9780470151808.sc02d08s20

Back

Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone

Journal article

Open access

Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone

Michelle H Theus, Jerome Ricard and Daniel J Liebl

Current protocols in stem cell biology, v Chapter 2(1), pp Unit 2D.8-2D.8.10

Mar 2012

DOI: https://doi.org/10.1002/9780470151808.sc02d08s20

PMID: 22415840

Files and links (1)

url

https://europepmc.org/articles/pmc3312298?pdf=renderView

Accepted (AM)Open Access (License Unspecified), Open

Abstract

Adult Stem Cells - cytology

Adult Stem Cells - metabolism

Animals

Cell Adhesion

Cell Culture Techniques - methods

Cell Differentiation

Cell Proliferation

Cell Separation

Cell Survival

Cells, Cultured

Cerebral Ventricles - cytology

Male

Mice

Neural Stem Cells - cytology

Neural Stem Cells - metabolism

Reproducibility of Results

RNA, Small Interfering - metabolism

Transfection

Endogenous neural stem/progenitor cells (NSPCs) residing in the subventricular zone (SVZ) of the adult mouse forebrain have been shown to enhance their neurogenic potential in response to CNS injury. Mechanisms involved in regulating adult neurogenesis under naïve or stressed conditions can be studied using a monolayer cell-culture system of the nestin-expressing NSPC lineage to analyze proliferation, survival, and differentiation. Here, a protocol for the expansion of NSPCs for studies aimed at understanding the functional role of NSPCs in maintaining adult neurogenic processes is described. This unit outlines detailed procedures for: (1) isolation, maintenance, and culture of the NSPC component of the SVZ niche from the lateral wall of the lateral ventricle; (2) characterization of NSPC functions by examining proliferation, survival, and differentiation; and (3) efficient siRNA transfection methods in 96-well format.

Metrics

5 Record Views

13 citations in Scopus

Details

Title: Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone
Creators: Michelle H Theus - University of Miami
Jerome Ricard
Daniel J Liebl - University of Miami
Publication Details: Current protocols in stem cell biology, v Chapter 2(1), pp Unit 2D.8-2D.8.10
Publisher: Wiley
Grant note: NS064699 / NINDS NIH HHS P50 NS030291-18 / NINDS NIH HHS P50 NS030291 / NINDS NIH HHS R01 NS049545 / NINDS NIH HHS T32 NS007459 / NINDS NIH HHS NS049545 / NINDS NIH HHS R01 NS049545-08 / NINDS NIH HHS F32 NS064699-02 / NINDS NIH HHS F32 NS064699 / NINDS NIH HHS NS30291 / NINDS NIH HHS T32 NS007459-10 / NINDS NIH HHS NS007459 / NINDS NIH HHS
Resource Type: Journal article
Language: English
Academic Unit: Biology
Scopus ID: 2-s2.0-84868144207
Other Identifier: 991021463582804721

Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone

Files and links (1)

Abstract

Metrics

Details

Drexel University Social media