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Journal article
Resistance of human hepatitis delta virus RNAs to dicer activity
Journal of virology, v 77(22), pp 11910-11917
01 Nov 2003
PMID: 14581527
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The endonuclease dicer cleaves RNAs that are 100% double stranded and certain RNAs with extensive but <100% pairing to release approximately 21-nucleotide (nt) fragments. Circular 1,679-nt genomic and antigenomic RNAs of human hepatitis delta virus (HDV) can fold into a rod-like structure with 74% pairing. However, during HDV replication in hepatocytes of human, woodchuck, and mouse origin, no approximately 21-nt RNAs were detected. Likewise, in vitro, purified recombinant dicer gave <0.2% cleavage of unit-length HDV RNAs. Similarly, rod-like RNAs of potato spindle tuber viroid (PSTVd) and avocado sunblotch viroid (ASBVd) were only 0.5% cleaved. Furthermore, when a 66-nt hairpin RNA with 79% pairing, the putative precursor to miR-122, which is an abundant liver micro-RNA, replaced one end of HDV genomic RNA, it was poorly cleaved, both in vivo and in vitro. In contrast, this 66-nt hairpin, in the absence of appended HDV sequences, was >80% cleaved in vitro. Other 66-nt hairpins derived from one end of genomic HDV, PSTVd, or ASBVd RNAs were also cleaved. Apparently, for unit-length RNAs of HDV, PSTVd, and ASBVd, it is the extended structure with <100% base pairing that confers significant resistance to dicer action.
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Details
- Title
- Resistance of human hepatitis delta virus RNAs to dicer activity
- Creators
- Jinhong Chang - Fox Chase Cancer CenterPatrick Provost - Université LavalJohn M Taylor
- Publication Details
- Journal of virology, v 77(22), pp 11910-11917
- Publisher
- American Society for Microbiology (ASM)
- Grant note
- R01 AI026522 / NIAID NIH HHS P30 CA006927 / NCI NIH HHS CA-06927 / NCI NIH HHS AI-26522 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000186294300005
- Scopus ID
- 2-s2.0-0242363258
- Other Identifier
- 991020547440304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Virology