Journal article
Role of G(alpha q) or G(alpha o) proteins in alpha(1)-adrenoceptor subtype mediated responses in Fischer 344 rat aorta
Molecular pharmacology, v 52(6), pp 1064-1070
01 Dec 1997
PMID: 9415716
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Abstract
Previous studies showed that alpha-adrenoceptor (AR) stimulation with norepinephrine is more potent at eliciting contraction in aortas from 1-month-old Fischer 344 rats than from older rats and that this response is mediated by alpha(1b)- and alpha(1d)-AR subtypes in 1-month-old rats. We examined the G proteins responsible for alpha(1)-AR-mediated contractile response and inositol phosphate accumulation in the aortas of 1-month-old Fischer 344 rats. Pertussis toxin (PTX) treatment (2.5 mu g/ml for 4 hr) of aortic rings partially inhibited phenylephrine (PHE)-stimulated contraction and inositol phosphate accumulation, suggesting the involvement of PTX-sensitive and -insensitive G proteins. Specific antisera directed against G(alpha q) and G(alpha o) but not G(alpha s) and G(alpha i) precipitated specific alpha(1)-AR binding sites labeled with 2-[beta-(4-hydroxy-3-[I-125]iodophenyl)ethylaminomethyl]tetralone. The number of 2-[beta-(4-hydroxy-3-[I-125] iodophenyl)ethylaminomethyl]tetralone binding sites precipitated by G(alpha) proteins was increased by activating membrane alpha(1)-ARs with PHE. Moreover, PHE stimulated the palmitoylation of G(alpha q) and G(alpha o), and this response was blocked by the alpha(1)-AR antagonist prazosin. Characterization of the alpha(1)-AR subtypes that couple to G proteins indicates that although aortic alpha(1a)- alpha(1b)- and alpha(1d)-ARs were associated with G(alpha q), alpha(1b)-AR was also linked to G(alpha o). These results suggest that alpha(1)-ARs mediate the contractile response in rat aorta by coupling to both G(q) protein and the PTX-sensitive G(o) protein.
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Details
- Title
- Role of G(alpha q) or G(alpha o) proteins in alpha(1)-adrenoceptor subtype mediated responses in Fischer 344 rat aorta
- Creators
- H Gurdal - Hahnemann University HospitalT M Seasholtz - United States Department of Veterans AffairsH Y Wang - United States Department of Veterans AffairsR D Brown - United States Department of Veterans AffairsM D Johnson - United States Department of Veterans AffairsEitan Friedman
- Publication Details
- Molecular pharmacology, v 52(6), pp 1064-1070
- Publisher
- Amer Soc Pharmacology Experimental Therapeutics
- Number of pages
- 7
- Grant note
- AG07700; AG14510; AG13282 / NIA NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA) P30AG013282 / NATIONAL INSTITUTE ON AGING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000071023400017
- Scopus ID
- 2-s2.0-0031468128
- Other Identifier
- 991019168311304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Pharmacology & Pharmacy