Journal article
Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments
The Journal of cell biology, v 168(5), pp 697-703
28 Feb 2005
PMID: 15728192
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Recent studies have shown that the transport of microtubules (MTs) and neurofilaments (NFs) within the axon is rapid, infrequent, asynchronous, and bidirectional. Here, we used RNA interference to investigate the role of cytoplasmic dynein in powering these transport events. To reveal transport of MTs and NFs, we expressed EGFP-tagged tubulin or NF proteins in cultured rat sympathetic neurons and performed live-cell imaging of the fluorescent cytoskeletal elements in photobleached regions of the axon. The occurrence of anterograde MT and retrograde NF movements was significantly diminished in neurons that had been depleted of dynein heavy chain, whereas the occurrence of retrograde MT and anterograde NF movements was unaffected. These results support a cargo model for NF transport and a sliding filament model for MT transport.
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Details
- Title
- Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments
- Creators
- Yan He - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129Franto Francis - Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, PA 19140Kenneth A Myers - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129Wenqian Yu - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129Mark M Black - Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, PA 19140Peter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129
- Publication Details
- The Journal of cell biology, v 168(5), pp 697-703
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000227461800007
- Scopus ID
- 2-s2.0-14744271078
- Other Identifier
- 991014877654204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology