Journal article
Role of natural killer and T-cells in interferon induced inhibition of spontaneous metastases of the B16F10L murine melanoma
Cancer research (Chicago, Ill.), v 51(4), pp 1124-1128
15 Feb 1991
PMID: 1705166
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have previously demonstrated that interferon (IFN) treatment of mice bearing the spontaneously metastasizing B16F10L murine melanoma on days -5 to -1 prior to surgical removal (day 0) of the primary tumor resulted in survival of greater than 50% of treated mice. The antitumor effect was correlated with an early increase of natural killer (NK) cell cytotoxicity followed by a later developing specific cytolytic T-cell response. The purpose of this study was to establish definitively the roles of NK, CD4, and CD8 cells as mediators of the antitumor/antimetastatic effects of IFN treatment by administration of anti-asialo-GM1, anti-L3T4, and/or anti-Lyt-2 antisera. Depletion of NK cells, alone or in combination with T-cells, eliminated the protective effect of IFN treatment. Depletion of both CD4 and CD8 cells, however, did not significantly alter the therapeutic effect of IFN therapy. Collectively, these data conclusively demonstrated the importance of NK cells as mediators of the IFN induced antitumor state. However, in mice depleted of CD4 cells alone, the protective effect of IFN was eliminated, in spite of the presence of intact NK cells. In vitro analysis of NK cytotoxicity on day 1 after surgery demonstrated (a) a lack of IFN induced stimulation of NK activity in CD4 depleted mice and (b) a significant increase in both baseline and IFN induced NK cytotoxicity in CD8 depleted mice. These data suggested a CD8 cell mediated inhibition of NK activity/stimulation in CD4 depleted mice, possibly responsible for the lack of response to IFN therapy in that group. These results demonstrate the importance of not only individual components of the immune system but also the interaction of these components in both the natural and IFN induced control of spontaneous B16F10L metastases.
Metrics
7 Record Views
Details
- Title
- Role of natural killer and T-cells in interferon induced inhibition of spontaneous metastases of the B16F10L murine melanoma
- Creators
- S N Markovic - Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19129D M Murasko
- Publication Details
- Cancer research (Chicago, Ill.), v 51(4), pp 1124-1128
- Grant note
- CA43386 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:A1991EX82700011
- Scopus ID
- 2-s2.0-0025804998
- Other Identifier
- 991020950433204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Oncology