Journal article
Roles of ChlR1 DNA helicase in replication recovery from DNA damage
Experimental cell research, v 319(14), pp 2244-2253
15 Aug 2013
PMID: 23797032
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The ChlR1 DNA helicase is mutated in Warsaw breakage syndrome characterized by developmental anomalies, chromosomal breakage, and sister chromatid cohesion defects. However, the mechanism by which ChlR1 preserves genomic integrity is largely unknown. Here, we describe the roles of ChlR1 in DNA replication recovery. We show that ChlR1 depletion renders human cells highly sensitive to cisplatin; an interstrand-crosslinking agent that causes stalled replication forks. ChlR1 depletion also causes accumulation of DNA damage in response to cisplatin, leading to a significant delay in resolution of DNA damage. We also report that ChlR1-depleted cells display defects in the repair of double-strand breaks induced by the I-PpoI endonuclease and bleomycin. Furthermore, we demonstrate that ChlR1-depeleted cells show significant delays in replication recovery after cisplatin treatment. Taken together, our results indicate that ChlR1 plays an important role in efficient DNA repair during DNA replication, which may facilitate efficient establishment of sister chromatid cohesion.
•ChlR1 depletion causes cellular sensitivity to cisplatin, an ICL agent.•ChlR1 depletion causes accumulation of DNA damage in response to cisplatin.•ChlR1-depleted cells display defects in DSB repair.•ChlR1 is involved in replication recovery after cisplatin treatment.•ChlR1 is involved in efficient DNA repair during DNA replication.
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Details
- Title
- Roles of ChlR1 DNA helicase in replication recovery from DNA damage
- Creators
- Niyant Shah - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15th Street, Philadelphia, PA 19102, USAAkira Inoue - Department of Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, TN 38105, USASeung Woo Lee - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15th Street, Philadelphia, PA 19102, USAKate Beishline - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15th Street, Philadelphia, PA 19102, USAJill M Lahti - Department of Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, TN 38105, USAEishi Noguchi - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15th Street, Philadelphia, PA 19102, USA
- Publication Details
- Experimental cell research, v 319(14), pp 2244-2253
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000322804900011
- Scopus ID
- 2-s2.0-84880760617
- Other Identifier
- 991014878430204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology
- Oncology