Journal article
Roles of p300, pocket proteins, and hTBP in E1A‐mediated transcriptional regulation and inhibition of p53 transactivation activity
Journal of cellular biochemistry, v 66(3)
01 Sep 1997
PMID: 9257185
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Abstract
The conserved region 1 and the extreme N‐terminus of adenoviral oncoprotein E1A are essential for transforming activity. They also play roles in the interaction of E1A with p300/CBP and pRb and are involved in both transactivation and repression of host gene expression. It was reported recently that p53‐mediated transactivation is specifically repressed by E1A and that p53‐induced apoptosis can be protected by pRb. In this report, we investigated the roles of pRb and p300 in the N‐terminus of E1A‐mediated transcriptional regulation. We demonstrate here that p300 and pRb have no effect on DBD.1‐70 transactivation and that overexpression of p300 or pRb failed to relieve the repression by E1A. Repression of p53 transactivation requires both the extreme amino terminus and CR1 but not CR2. This repressive activity of E1A specifically correlates with E1A's ability to bind p300 and TBP. On the other hand, E1A inhibited the transactivation activity of a fusion construct containing the DNA binding domain of yeast Gal4 and the transactivation domain of p53. When p53 was cotransfected with E1A, similar inhibition was found in Saos‐2 cells that lack endogenous pRb and p53 activity. Introduction of pRb into Saos‐2 cells did not affect p53 transcription activity. E1A‐mediated repression can be relieved by overexpression of either p300, hTBP, or TFIIB but cannot be released by overexpression of pocket proteins. Our data suggest that p300/CBP and TBP but not the pocket proteins, pRb, p107, and pRb2/p130 are functional targets of E1A in transcriptional regulation and that p53 transactivation requires the function of the p300/TBP/TFIIB complex, thus delineating a new pathway by which E1A may exert its transforming activity. J. Cell. Biochem. 66:277–285, 1997. © 1997 Wiley‐Liss, Inc.
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Details
- Title
- Roles of p300, pocket proteins, and hTBP in E1A‐mediated transcriptional regulation and inhibition of p53 transactivation activity
- Creators
- Nianli SangMaria Laura AvantaggiatiAntonio Giordano
- Publication Details
- Journal of cellular biochemistry, v 66(3)
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- Number of pages
- 9
- Grant note
- Sbarro Institute for Cancer Research and Molecular Medicine, The Council for Tobacco Research NIH (RO1CA 60999‐01; PO1 NS36466)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:A1997XN91800001
- Scopus ID
- 2-s2.0-0030753187
- Other Identifier
- 991014877802104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology