Journal article
Rolling adhesion kinematics of yeast engineered to express selectins
Biotechnology progress, v 19(3), pp 1033-1037
01 May 2003
PMID: 12790675
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Selectins are cell adhesion molecules that mediate capture of leukocytes on vascular endothelium as an essential component of the inflammatory response. Here we describe a method for yeast surface display of selectins, together with a functional assay that measures rolling adhesion of selectin-expressing yeast on a ligand-coated surface. E-selectin-expressing yeast roll specifically on surfaces bearing sialyl-Lewis-x ligands. Observation of yeast rolling dynamics at various stages of their life cycle indicates that the kinematics of yeast motion depends on the ratio of the bud radius to the parent radius (B/P). Large-budded yeast "walk" across the surface, alternately pivoting about bud and parent. Small-budded yeast "wobble" across the surface, with bud pivoting about parent. Tracking the bud location of budding yeast allows measurement of the angular velocity of the yeast particle. Comparison of translational and angular velocities of budding yeast demonstrates that selectin-expressing cells are rolling rather than slipping across ligand-coated surfaces.
Metrics
Details
- Title
- Rolling adhesion kinematics of yeast engineered to express selectins
- Creators
- Sujata K Bhatia - University of PennsylvaniaJeffrey S Swers - Massachusetts Institute of TechnologyRaymond T CamphausenK Dane Wittrup - Massachusetts Institute of TechnologyDaniel A Hammer - University of Pennsylvania
- Publication Details
- Biotechnology progress, v 19(3), pp 1033-1037
- Publisher
- American Chemical Society; Washington, DC
- Grant note
- HL18208 / NHLBI NIH HHS GM59100 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000186413200050
- Scopus ID
- 2-s2.0-0038458847
- Other Identifier
- 991021229984504721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Food Science & Technology