Journal article
RrS1-like Sequences of Water Frogs from Central Europe and Around the Aegean Sea: Chromosomal Organization, Evolution, Possible Function
Journal of molecular evolution, v 72(4), pp 368-382
2011
PMID: 21424546
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
RrS1
-like sequences of water frogs (genus
Pelophylax
) display varied genomic organization, whereas the centromeric hybridization pattern reveals species-specific differences. Using fluorescent in situ hybridization,
Pelophylax
cf.
bedriagae
,
Pelophylax
kurtmuelleri
, and
Pelophylax
ridibundus
showed a hybridization signal at centromeres of chromosomes 1–5, but in
P
.
kurtmuelleri
the medium-small chromosome labeled was 10 rather than 8.
Pelophylax
cretensis
had almost 16 of 26 centromeres labeled, as did
Pelophylax
lessonae
from Poland when its chromosomes are hybridized with a homologous probe. When
Stu
I-digested genomic DNA was hybridized with
RrS1
probe, hybridization ladders for
P
.
ridibundus
from Poland have evenly spaced steps (about 100 bp) of uniform intensity from about 200 bp upward. Steps in hybridization ladders from circum-Aegean taxa vary in intensity: larger, odd-numbered steps are often fainter. A strong double band (800/900 bp) in Anatolian
P
. cf.
bedriagae
, emphasized by a weak 700 bp band, distinguishes them from
P
.
kurtmuelleri
from the Peloponnisos, in which the 900 bp band is almost absent. The ladder in
P
.
cretensis
lacks odd-numbered steps. A and B repeats, observed originally within the
RrS1
satellite of
P
.
ridibundus
, occur also in the circum-Aegean frogs and in
P
.
lessonae
,
Pelophylax
epeiroticus
,
Pelophylax
saharicus
, and
Pelophylax
shqipericus
. It is plausible that AB dimers or ABB trimers rather than A or B monomers correspond to functional/evolutionary units. The presence of regions similar to yeast CDEs and mammalian CENP-B boxes suggests a role for
RrS1
sequences in centromere organization.
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Details
- Title
- RrS1-like Sequences of Water Frogs from Central Europe and Around the Aegean Sea: Chromosomal Organization, Evolution, Possible Function
- Creators
- Silvia Marracci - University of PisaValentina Michelotti - University of PisaGaston-Denis Guex - University of ZurichHansjürg Hotz - University of ZurichThomas Uzzell - Systematic (Netherlands)Matilde Ragghianti - University of Pisa
- Publication Details
- Journal of molecular evolution, v 72(4), pp 368-382
- Publisher
- Springer-Verlag
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000290578400003
- Scopus ID
- 2-s2.0-79958204965
- Other Identifier
- 991019350672204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Evolutionary Biology
- Genetics & Heredity