Journal article
SARS-CoV-2 infection of African green monkeys results in mild respiratory disease discernible by PET/CT imaging and shedding of infectious virus from both respiratory and gastrointestinal tracts
PLoS pathogens, v 16(9), 1008903
18 Sep 2020
PMID: 32946524
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Vaccines are urgently needed to combat the global coronavirus disease 2019 (COVID-19) pandemic, and testing of candidate vaccines in an appropriate non-human primate (NHP) model is a critical step in the process. Infection of African green monkeys (AGM) with a low passage human isolate of SARS-CoV-2 by aerosol or mucosal exposure resulted in mild clinical infection with a transient decrease in lung tidal volume. Imaging with human clinical-grade 18F-fluoro-2-deoxy-D-glucose positron emission tomography (18F-FDG PET) co-registered with computed tomography (CT) revealed pulmonary lesions at 4 days post-infection (dpi) that resolved over time. Infectious virus was shed from both respiratory and gastrointestinal (GI) tracts in all animals in a biphasic manner, first between 2-7 dpi followed by a recrudescence at 14-21 dpi. Viral RNA (vRNA) was found throughout both respiratory and gastrointestinal systems at necropsy with higher levels of vRNA found within the GI tract tissues. All animals seroconverted simultaneously for IgM and IgG, which has also been documented in human COVID-19 cases. Young AGM represent an species to study mild/subclinical COVID-19 disease and with possible insights into live virus shedding. Future vaccine evaluation can be performed in AGM with correlates of efficacy being lung lesions by PET/CT, virus shedding, and tissue viral load.
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Details
- Title
- SARS-CoV-2 infection of African green monkeys results in mild respiratory disease discernible by PET/CT imaging and shedding of infectious virus from both respiratory and gastrointestinal tracts
- Creators
- Amy L Hartman - University of PittsburghSham Nambulli - University of PittsburghCynthia M McMillen - University of PittsburghAlexander G White - University of PittsburghNatasha Louise Tilston-Lunel - University of PittsburghJoseph R Albe - University of PittsburghEmily Cottle - University of PittsburghMatthew D Dunn - University of PittsburghL James Frye - University of PittsburghTheron H Gilliland - University of PittsburghEmily L Olsen - University of PittsburghKatherine J O'Malley - University of PittsburghMadeline M Schwarz - University of PittsburghJaime A Tomko - University of PittsburghReagan C Walker - University of PittsburghMengying Xia - University of PittsburghMatthew S Hartman - Allegheny Health NetworkEdwin Klein - University of PittsburghCharles A Scanga - University of PittsburghJoAnne L Flynn - University of PittsburghWilliam B Klimstra - University of PittsburghAnita K McElroy - University of PittsburghDouglas S Reed - University of PittsburghW Paul Duprex - University of Pittsburgh
- Publication Details
- PLoS pathogens, v 16(9), 1008903
- Publisher
- Public LIbrary of Science (PLOS)
- Grant note
- R01 AI150792 / NIAID NIH HHS P40 OD010965 / NIH HHS R01 AI161765 / NIAID NIH HHS T32 AI060525 / NIAID NIH HHS R01 NS101100 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Radiology (Radiologic Sciences)
- Web of Science ID
- WOS:000573820300001
- Scopus ID
- 2-s2.0-85092022235
- Other Identifier
- 991021897327204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Microbiology
- Parasitology
- Virology