Journal article
SNR1 is an essential subunit in a subset of drosophila brm complexes, targeting specific functions during development
Developmental biology, v 253(2)
2003
PMID: 12645932
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The
snr1 gene of
Drosophila melanogaster encodes a conserved component of the multiprotein Brahma (Brm) complex, a counterpart to the SWI/SNF complexes that participate in ATP-dependent chromatin remodeling. Loss-of-function and null mutations in the
snr1 gene reveal its essential role in
Drosophila development. We identified new mutant alleles and ectopically expressed deleted forms to dissect the specific functions of SNR1. Somatic and germ cell clone analyses confirmed its requirement in a continuous and widespread fashion for proper cell fate determination and oogenesis. Expression of SNR1 transgenes revealed unexpected roles in wing patterning, abdomen development, oogenesis, and sustained adult viability. A widespread distribution of SNR1 and BRM on the salivary gland polytene chromosomes showed that the Brm complex associated with many genes, but not always at transcribed loci, consistent with genetic data suggesting roles in both gene activation and repression. Despite essential Brm complex functions in leg development, genetic and protein localization studies revealed that
snr1 was not required or expressed in all tissues dependent on Brm complex activities. Thus, SNR1 is essential for some, but not all Brm functions, and it likely serves as an optional subunit, directing Brm complex activity to specific gene loci or cellular processes.
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Details
- Title
- SNR1 is an essential subunit in a subset of drosophila brm complexes, targeting specific functions during development
- Creators
- Claudia B Zraly - Department of Biology, Syracuse University, Syracuse, NY 13244-1270, USADaniel R Marenda - Department of Biology, Syracuse University, Syracuse, NY 13244-1270, USARunjhun Nanchal - Department of Biology, Syracuse University, Syracuse, NY 13244-1270, USAGiacomo Cavalli - Institut de Génétique Humaine, CNRS, Montpellier 34396, FranceChristian Muchardt - Expression Génétique et Maladies, URA 1644 du CNRS, Département Biologie du Développement, Institut Pasteur, Paris, FranceAndrew K Dingwall - Department of Biology, Syracuse University, Syracuse, NY 13244-1270, USA
- Publication Details
- Developmental biology, v 253(2)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000180688400011
- Scopus ID
- 2-s2.0-0037440460
- Other Identifier
- 991014877836904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Developmental Biology